Clamp Loader Complex - University of California, Berkeley
David Jeruzalmi, Mike O'Donnell, and John Kuriyan. Cell, Vol 106, 429-441, August 2001 Summary The gamma complex, an AAA+ ATPase, is the bacterial homolog of eukaryotic Replication Factor C (RFC) that loads the sliding clamp (beta, homologous to PCNA) onto DNA. The 2.7/3.0 Å crystal structure of gamma complex reveals a pentameric arrangement
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Clamp loaders and sliding clamps | Request PDF
Mike O'Donnell. The γ complex (γδδ′χψ) and τ complex (τδδ′χψ) clamp loaders require ATP hydrolysis to load β sliding clamps onto DNA. The β sliding clamp tethers the polymerase
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Clamp loaders and sliding clamps - PubMed
Clamp loaders and sliding clamps Curr Opin Struct Biol. 2002 Apr;12(2):217-24. doi: 10.1016/s0959-440x(02)00313-5. Authors David Jeruzalmi 1, Mike O'Donnell, John Kuriyan. Affiliation 1 Department of Molecular and Cell Biology, Howard Hughes Medical Institute
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Structural analysis of a eukaryotic sliding DNA clamp
Sliding clamps are ring-shaped proteins that encircle DNA and confer high processivity on DNA polymerases. Here we report the crystal structure of the five-protein clamp loader complex
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sliding clamps #LOCANEX
@article{Jeruzalmi2002ClampLA, title={Clamp loaders and sliding clamps.}, author={D. Jeruzalmi and M. O'Donnell and J. Kuriyan}, journal={Current opinion A coherent view of the structure and function of DNA polymerase processivity factors (sliding …
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Clamp loaders and sliding clamps - ScienceDirect
Abstract. A coherent view of the structure and function of DNA polymerase processivity factors (sliding clamps and clamp loaders) is emerging from recent structural studies. Crystal structures of sliding clamps from the T4 and RB69 bacteriophages, and from an archaebacterium expand the gallery of ring-shaped processivity factors and clarify how
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sliding clamps #LOCANEX
@article{Jeruzalmi2002ClampLA, title={Clamp loaders and sliding clamps.}, author={D. Jeruzalmi and M. O'Donnell and J. Kuriyan}, journal={Current opinion A coherent view of the structure and function of DNA polymerase processivity factors (sliding …
Get a quoteStructural analysis of a eukaryotic sliding DNA clamp
Jun 17, 2004 · The efficiency of lagging strand synthesis is increased by the ability of clamp loaders to position sliding clamps such that they are oriented
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Clamp loading, unloading and intrinsic stability of the
The clamp loaders (RF-C and γ complex) were also capable of unloading their respective clamps from DNA in the presence of ATP. Conclusions: The stability of the different clamps in solution correlates with their stability on DNA. Thus, the low stability of the T4 clamp explains the inability to isolate gp45 on DNA.
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Clamp loader structure predicts the architecture of DNA
Fig. 1 Clamp loaders assemble clamps onto DNA for use by other enzymes. (a) The scheme illustrates the need for a clamp loader to open and close the circular clamp, placing it around DNA in an ATP dependent reaction.The clamp loader leaves, allowing other enzymes, such as polymerase, to target the clamp for processive synthesis. (b) Sliding clamps have similar …
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Structure of a Sliding Clamp on DNA: Cell
Jan 11, 2008 · The structure of the E. coli β clamp polymerase processivity factor has been solved in complex with primed DNA. Interestingly, the clamp directly binds the DNA duplex and also forms a crystal contact with the ssDNA template strand, which binds into the protein-binding pocket of the clamp. We demonstrate that these clamp-DNA interactions function in clamp …
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Introduction - University of California, Berkeley
David Jeruzalmi, Mike O'Donnell, and John Kuriyan. The sliding clamps and their associated clamp loading systems are of broad importance in many cellular processes involving DNA, delta-prime and delta subunits, at 2.7/3.0 Å resolution. The clamp loader complex is assembled from five subunits, each with the same fold. Nevertheless, each
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Clamp loading, unloading and intrinsic stability of the
The clamp can not assemble itself on DNA. Instead, a protein complex known as a clamp loader utilizes ATP to assemble the ring around the primer-template. This study compares properties of the human PCNA clamp with those of E. coli and T4 phage. Results: The PCNA ring is a stable trimer down to a concentration below 100 nM (K d ≈ 21 nM).
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BioKDE
Jan 10, 2021 · Clamp loaders and sliding clamps. David Jeruzalmi, Mike O'Donnell, John Kuriyan.
Get a quoteClamp loader structure predicts the - O'Donnell Lab …
Mike O'Donnell†, David Jeruzalmi and John Kuriyan shaped sliding DNA clamp, and a clamp loader complex (reviewed in [1–3]). The multisubunit clamp loader couples Clamp loaders assemble clamps onto DNA for use by other enzymes. (a) The scheme illustrates the need for a clamp loader to open
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Clamp loaders and sliding clamps. - Abstract - Europe PMC
Apr 01, 2002 · Assembly of a chromosomal replication machine: two DNA polymerases, a clamp loader, and sliding clamps in one holoenzyme particle. IV. ATP-binding site mutants identify the clamp loader. Xiao H, Naktinis V, O'Donnell M. J Biol …
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[PDF] Clamp loaders and sliding clamps. | Semantic Scholar
Apr 01, 2021 · Crystallographic and electron microscopic views of clamp loaders from bacteria, archaebacteria and eukaryotes emphasize their common architecture and have produced models of how ATPbinding might be coupled to clamp opening/loading. A coherent view of the structure and function of DNA polymerase processivity factors (sliding clamps and clamp loaders) is …
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Clamp loader structure predicts the architecture of DNA
By Mike O'Donnell, David Jeruzalmi and John Kuriyan. Cite . BibTex; we discuss the structures of δ–β and the γ3δδ′ complex and its mechanism of action as a clamp loader of the E. coli β sliding clamp. We also expand upon the implications of the structural findings to the structure and function of the eukaryotic clamp loader, RFC
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Mechanism of processivity clamp opening by the delta
pmid: 11525728, nlmid 0413066, doi: Mechanism of processivity clamp opening by the delta subunit wrench of the clamp loader complex of E. coli DNA polymerase III. Jeruzalmi, D Yurieva, O Zhao, Y Young, M Stewart, J Hingorani, M O'Donnell, M Kuriyan, J
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Apr 01, 2002 · Two new structures of sliding clamps were determined recently, those of gp45 from bacteriophages T4 and RB69 8••., 10•., and PCNA from the archaebacterium Pyrococcus furiosus ().These structures have the same modular and highly symmetric design seen originally in the bacterial and eukaryotic sliding clamps 13., 14., 15..The structure of the sliding clamp …
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